Fig. 1
Characterization of P-ASCs and H-ASCs. Human ASCs were cultured under physioxia (2% O2, P-ASCs) or hyperoxia (20% O2, H-ASCs) for the entire in vitro period until assayed at passage 3. a Flow cytometry was applied to demonstrate the immunophenotype of P-ASCs and H-ASCs. b Morphology of ASCs cultured in α-modified Eagle’s medium (α-MEM) with 10% fetal bovine serum (FBS). c After inducing adipogenesis for 7 days, lipid clusters were stained by oil red O. d Oil red O staining was quantified by the ratio of oil red O+ area to total image area for three fields. Data are presented as the mean ± SD, *P > 0.05. e Western blot of hypoxia-inducible factor 1 (HIF-1) and β-actin (reference gene) expression. Scale bar = 50 μm. ASCs adipose-derived stem cells, H-ASCs hyperoxia ASCs, P-ASCs physioxia ASCs