Fig. 5
Physioxia improved ASC survivability under ischemic conditions. ASCs (1 × 104) were seeded onto 96-well plates and incubated in four hostile environments for 24 h: (a) ischemic model, 1% O2, pH 6.4 and 0.56 μM glucose; (b) hypoxic model, 1% O2, pH 7.4 and 5.6 μM glucose; (c) acidic model, 20% O2, pH 6.4 and 5.6 μM glucose; (d) nutrient-depleted model, 20% O2, pH 7.4 and 0.56 μM glucose. (A) Fluorescent images showing the cell death rate by live/dead cell staining. The cell death rate was obtained by the ratio of dead cells to total cells. Three fields were quantified. (B) The cell survival rate was detected by WST-8 presented as the ratio of OD24 to OD0. Data are presented as the mean ± SD, *P < 0.05, **P < 0.01, Student’s t tests, scale bar = 200 μm. ASCs adipose-derived stem cells, H-ASCs hyperoxia ASCs, OD0, optical density value at 0 h, OD24, optical density value at 24 h, P-ASCs, physioxia ASCs