Fig. 2
From: Melatonin rescued interleukin 1β-impaired chondrogenesis of human mesenchymal stem cells
MLT rescued IL-1β-impaired matrix accumulation during chondrogenesis process. Alcian blue staining performed to visualize matrix accumulation. Pellets of different stages harvested. Paraformaldehyde 4% used for fixation. Paraffin sections (4 μm thick) prepared for staining. Cartilage pellets cultured for 7 (a), 14 (c), and 21(e) days observed by microscope in representative samples. Scale bars: 0.1 mm. Quantitative analysis of glycosaminoglycan (GAG) evaluated typical matrix accumulation during chondrogenesis. GAG content quantitatively examined and normalized by DNA content on days 7 (b), 14 (d), and 21 (f). Outcomes representative of three separate experiments. g Changing pattern of GAG/DNA from day 7 to day 21 in different groups. aP < 0.05 versus control group; bP < 0.05 versus IL-1β group. IL-1β interleukin-1β, MLT melatonin