Fig. 5
From: Melatonin rescued interleukin 1β-impaired chondrogenesis of human mesenchymal stem cells
MLT protected human MSCs from IL-1β-induced apoptosis in process of chondrogenesis. TUNEL assay showed apoptotic MSCs (green fluorescence) with PI-labeled nuclei (blue fluorescence) on days 7 (a), 14 (c), and 21 (e). Positive rates statistically analyzed on right correspondingly (b, d, f), and data represent mean ± SD of three independent experiments. Scale bars = 50 μm. aP < 0.05 versus control group; bP < 0.05 versus IL-1β group. DAPI 4′,6-diamidino-2-phenylindole, IL-1β interleukin-1β, MLT melatonin, TUNEL terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling