Fig. 4

Flow cytometry and fluorescence microscopy images of primary human dermal fibroblasts kept in culture for 2 months after RGB marking at different MOI values. MOI 1, 2, 3 or 10 used to transduce primary human dermal fibroblasts. a Flow cytometry at passage 2 showing homogeneous polyclonal state of four independent cultures. Microscopic images taken at passage 4. b Flow cytometry at passage 16 showing oligoclonal state with few very dominant clones present at all four MOIs. Microscopic images taken at passage 15, dominant clones clearly identifiable by colour. Insets display higher magnification. Refer to Additional file 1: Figure S4 for a full data set from the four independent fibroblast cultures transduced at different MOI values. BFP blue fluorescent protein, MOI multiplicity of infection, p passage, Q quadrant