Stem Cell Research & Therapy

Table 1 Sequence-specific primers

From: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

Gene		Sequence (5′–3′)
BMP2	Forward	TCTTCCGGGAACAGATACAGG
BMP2	Reverse	TGGTGTCCAATAGTCTGGTCA
BMPR1A	Forward	GGCCATTGCTTTGCCATTATAG
BMPR1A	Reverse	CTTTCGGTGAATCCTTGCATTG
BMPR1B	Forward	CCGACCTCGGTACAGCATTG
BMPR1B	Reverse	GCTCTGAGACTGCTCGATCAAG
BMPR2	Forward	TTGGGATAGGTGAGAGTCGAAT
BMPR2	Reverse	TGTTTCACAAGATTGATGTCCCC
Msx2	Forward	GGAGCACCGTGGATACAGG
Msx2	Reverse	TAGAAGCTGGGATGTGGTGAA
Runx2	Forward	ATGCTTCATTCGCCTCACAAA
Runx2	Reverse	GCACTCACTGACTCGGTTGG
osteocalcin	Forward	TGTCTTCTCCACAGCCTTCATG
osteocalcin	Reverse	ACCACTCCAGCACAACTCCTTC
GAPDH	Forward	ACCACAGTCCATGCCATCAC
GAPDH	Reverse	TCCACCACCCTGTTGCTGTA

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ISSN: 1757-6512

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