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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: miR-10a rejuvenates aged human mesenchymal stem cells and improves heart function after myocardial infarction through KLF4

Fig. 2

Overexpression of miR-10a in old hBM-MSCs decreased hypoxia-induced apoptosis and increased cell survival. miR-10a transduced into old hBM-MSCs by lentiviral vector (O-10a). Control vector-transduced old hBM-MSCs (O-c) served as control. Cells cultured for 72 h under hypoxia conditions. a Cell apoptosis assayed by TUNEL staining. Percentage of apoptotic cells (TUNEL+) quantified in O-c and O-10a hBM-MSCs. b Cell survival evaluated in O-c and O-10a hBM-MSCs. c Protein expression of MCL1 and PUMA evaluated by western blot analysis in O-c and O-10a hBM-MSCs. d Ratio of Bax/BCL2 quantified in O-c and O-10a hBM-MSCs. e Protein expression of cleaved caspase-3 and inhibitor of caspase-activated DNase (ICAD) assayed in O-c and O-10a hBM-MSCs. f Caspase-3 activity measured in O-c and O-10a hBM-MSCs. n = 6/group. Mean ± SD. *P < 0.05. DAPI 4′,6-diamidino-2-phenylindole, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling, RFU relative fluorescence units

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Stem Cell Research & Therapy

ISSN: 1757-6512