Fig. 3
iPSC-MSCs regulated cytokine production in unstimulated PBMCs. a IL-10 secretion levels in PBMCs under different culture conditions determined by ELISA (n = 6). b IFN-γ secretion levels in PBMCs under different culture conditions determined by ELISA (n = 6). c TH2-type cytokine (IL-4, IL-5, and IL-13) secretion levels in PBMCs under different culture conditions determined by ELISA (n = 6). d IL-4, IL-5, and IL-13 mRNA expression levels were decreased after coculture with iPSC-MSCs determined by qPCR (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, by Kruskal–Wallis rank-sum test followed by Mann–Whitney U test for two-group comparisons for (a), (b), by one-way ANOVA and Dunnett T3 test for multiple comparisons for (c), and by independent t test for (d). AR allergic rhinitis, BM-MSC bone marrow-derived mesenchymal stem cell, IFN-γ interferon gamma, IL interleukin, iPSC-MSC induced pluripotent stem cell-derived mesenchymal stem cell, Normal healthy donors, PBMC peripheral blood mononuclear cell