Fig. 5

Human iPSC-MSCs inhibited the differentiation of human Th1, Th2 and Th17 cells in vitro. a Representative gating strategies of flow cytometry analysis for T helper cells. b-d Representative dot plots showing the percentages of Th1 (b), Th2 (c) and Th17 (d) cells in CD4⁺ T cells cultured in different T cells differentiation medium with or without iPSC-MSCs. e-g Statistical analysis showing that the percentages of Th1(E), Th2 (f) and Th17 (g) cells could be significantly reduced by iPSC-MSCs. **P < 0.01 by the Mann-Whitney U test. Abbreviation: iPSC-MSCs induced pluripotent stem cell-derived mesenchymal stem cells. n = 5 for each group