Fig. 8

Flow cytometry graphs of MION-labelled MSCs isolated from tissue segments from two sheep at 1 h (a–g) and 24 h (h–l) after implantation. Tissue segments were dissected and adherent cells released by mild enzymatic digestion. a, k Distal lateral DDFT (segment A). b Distal medial DDFT (segment B). c, j Distal lateral SDFT (segment F). d, l Distal medial SDFT (segment G). e, h SDFT bifurcation (segment J). f Ventral synovium (segment K). g, i Sesamoid bones, distal to segment K. MION-labelled cells were detectable in segment J of both sheep at 1 h (e) and 24 h (h) post implantation. Cells were detectable at the level of proximal sesamoid bones (distal to segment K) in both samples at 1 h (g) but not at 24 h (i) post implantation. DDFT segments did not display MION-related fluorescence at either time point in both samples nor where cells were recovered from the synovial cavity (sample L). Sample identities are elaborated in Fig. 1 accompanying table