Fig. 5

BEX1 has no impact on hepatic differentiation of LPCs. a Clonogenic colony-forming assay of cultured LPCs from WT and Bex1^−/− mice established. Number of colonies quantified (mean ± SEM, n = 4, *P < 0.05, Mann–Whitney U test). b Clonogenic colony-forming assay of shNC and shBex1 LEPCs established. Number of colonies quantified (mean ± SEM, n = 4, *P < 0.05, Mann–Whitney U test). c LPCs from WT and Bex1^−/− mice cultured in hepatic differentiation medium for 7 days and subjected to PAS staining. Scale bars, 1000 μm. Results representative of three independent experiments with similar results. d Real-time PCR analysis of G6pc, Alb, Krt19 and Krt7 expression (mean ± SEM, n = 5, ^NSP > 0.05, Mann–Whitney U test). ALB albumin, BEX1 brain-expressed X-linked 1, G-6-Pase glucose 6-phosphatase, NS not significant, PAS periodic acid–Schiff, shBex1 lentivirus expressing shRNA specific to BEX1, shNC lentivirus expressing scrambled shRNA, WT wild-type