Fig. 5
From: Use of bioreactors for culturing human retinal organoids improves photoreceptor yields
Ultrastructural and topographical analysis of human hPSC-derived neuroepithelia and photoreceptor cells at early stages of development. a Diagrammatic representation of key structures of mature photoreceptor. b Immunohistochemical staining of cone photoreceptor (L/M-OPSIN, magenta) and its ribbon synapse (RIBEYE, green, arrow). c TEM image illustrating morphology of hPSC-derived photoreceptor OLM (arrows), IS, CC and OS-like structures. d SEM image from etched resin section 2 μm thick showing IS, CC and OS. e TEM images illustrating internal organisation of CC (high magnification box) and OS. f Backscatter EM images from different levels of 3view dataset showing photoreceptor ribbon synapse. g 3view sequence of backscatter EM images of hPSC-derived photoreceptor OS (yellow), CC (green) and IS (red). See Additional file 2: Movie 1. h 3view 3D reconstruction of 150 sections with thickness of 100 nm each. Representative photoreceptor OS, CC and IS observed in yellow, green and red respectively. CC connecting cilium, IS inner segment, OLM outer limiting membrane, OS outer segment