Table 1 Primers for target genes
Gene | Forward sequence | Reverse sequence |
|---|---|---|
Collagen Iα1 | 5′-CCTGGAAAGAATGGAGATGATG-3′ | 5′-ATCCAAACCACTGAAACCTCTG-3′ |
Collagen IIα1 | 5′-GGTAAGTGGGGCAAGACTGTTA-3′ | 5′-TGTTGTTTCTGGGTTCAGGTTT-3′ |
Sox-9 | 5′-GCCTCTACTCCACCTTCACCTA-3′ | 5′-GCTGTGTGTAGACAAGTTGTT-3′ |
Aggrecan | 5′-GTCAGATACCCCATCCACACTC-3′ | 5′-CATAAAAGACCTCACCCTCCAT-3′ |
LPL | 5’-TCCGCGTGATTGCAGAGAGAG-3′ | 5’-TGCTGCTTCTTTTGGC TCTGACT-3′ |
PPAR-2 | 5’-CGAGGGCGATCTTGACAGGAA − 3′ | 5’-CAGGGGGGTGATGT GTTTGAAC- 3′ |
RUNX-2 | 5’-ACGACAACCGCACCATGGT-3’ | 5’-CTGTAATCTGACTCT GTCCT-3’ |
ALP | 5’-TGGAGCTTCAGA AGCTCAACACCA-3’ | 5’-ATCTCGTTGTCTGAG TACCAGTCC-3’ |
p16 | 5′-ACCAGAGGCAGTAACCATGC-3′ | 5′-TGATCTAAGTTTCCCGAGGTTT-3′ |
p21 | 5′-TTAGCAGCGGAACAAGGAGT-3′ | 5′-CGTTAGTGCCAGGAAAGACA-3′ |
p53 | 5′-TAGTGTGGTGGTGCCCTATG-3′ | 5′-CCAGTGTGATGATGGTGAGG-3′ |
IL-6 | 5′-ATGCCTGACCTCAACTCCACT-3′ | 5′-GCCACCCAGCTGCAAGATTTC-3′ |
ADAMTS-5 | 5′-GGACCTACCACGAAAGCAGATC-3′ | 5′-GCCGGGACACACGGAGTAC-3′ |
MMP-13 | 5′-TGGAAGGATGCCTTTTTTTCTC-3′ | 5′-CACCCTCCCCAAGTATCAATAGG-3′ |
Brachyury | 5′-AGACAGCCAGCAATCTG-3′ | 5′-TGGAGGGAAGTGAGAGG-3′ |
KRT18 | 5′-GGACAGCTCTGACTCCAGGT-3′ | 5′-AGCTTGGAGAACAGCCTGAG-3′ |
HIF-1α | 5′-GAAGTGTACCCTAACTAGCCGAGGA-3′ | 5′-TGAATGTGGCCTGTGCAGTG-3′ |
CA12 | 5′-CTGTGGGTCCAGCTTTGGAA-3′ | 5′-TGCCATGCAGCAGAGTTAGGAG-3′ |
FOX1 | 5′-CACACAGGAATTCTGCTGAGGT-3′ | 5′-TGATTGGTCTCACATGTTTGCT-3′ |
PAX1 | 5′-AACTTGGCTTAAATCTCTGCTCCAC-3′ | 5′-GCATTGAAGGCTACATTTCACAGAC-3′ |
β-actin | 5′-GTGGGGCGCCCCAGGCACCA-3′ | 5′-CTTCCTTAATGTCAC GCACGATTTC-3′ |