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Table 1 Primers for target genes

From: Co-culturing nucleus pulposus mesenchymal stem cells with notochordal cell-rich nucleus pulposus explants attenuates tumor necrosis factor-α-induced senescence

Gene Forward sequence Reverse sequence
Collagen Iα1 5′-CCTGGAAAGAATGGAGATGATG-3′ 5′-ATCCAAACCACTGAAACCTCTG-3′
Collagen IIα1 5′-GGTAAGTGGGGCAAGACTGTTA-3′ 5′-TGTTGTTTCTGGGTTCAGGTTT-3′
Sox-9 5′-GCCTCTACTCCACCTTCACCTA-3′ 5′-GCTGTGTGTAGACAAGTTGTT-3′
Aggrecan 5′-GTCAGATACCCCATCCACACTC-3′ 5′-CATAAAAGACCTCACCCTCCAT-3′
LPL 5’-TCCGCGTGATTGCAGAGAGAG-3′ 5’-TGCTGCTTCTTTTGGC TCTGACT-3′
PPAR-2 5’-CGAGGGCGATCTTGACAGGAA − 3′ 5’-CAGGGGGGTGATGT GTTTGAAC- 3′
RUNX-2 5’-ACGACAACCGCACCATGGT-3’ 5’-CTGTAATCTGACTCT GTCCT-3’
ALP 5’-TGGAGCTTCAGA AGCTCAACACCA-3’ 5’-ATCTCGTTGTCTGAG TACCAGTCC-3’
p16 5′-ACCAGAGGCAGTAACCATGC-3′ 5′-TGATCTAAGTTTCCCGAGGTTT-3′
p21 5′-TTAGCAGCGGAACAAGGAGT-3′ 5′-CGTTAGTGCCAGGAAAGACA-3′
p53 5′-TAGTGTGGTGGTGCCCTATG-3′ 5′-CCAGTGTGATGATGGTGAGG-3′
IL-6 5′-ATGCCTGACCTCAACTCCACT-3′ 5′-GCCACCCAGCTGCAAGATTTC-3′
ADAMTS-5 5′-GGACCTACCACGAAAGCAGATC-3′ 5′-GCCGGGACACACGGAGTAC-3′
MMP-13 5′-TGGAAGGATGCCTTTTTTTCTC-3′ 5′-CACCCTCCCCAAGTATCAATAGG-3′
Brachyury 5′-AGACAGCCAGCAATCTG-3′ 5′-TGGAGGGAAGTGAGAGG-3′
KRT18 5′-GGACAGCTCTGACTCCAGGT-3′ 5′-AGCTTGGAGAACAGCCTGAG-3′
HIF-1α 5′-GAAGTGTACCCTAACTAGCCGAGGA-3′ 5′-TGAATGTGGCCTGTGCAGTG-3′
CA12 5′-CTGTGGGTCCAGCTTTGGAA-3′ 5′-TGCCATGCAGCAGAGTTAGGAG-3′
FOX1 5′-CACACAGGAATTCTGCTGAGGT-3′ 5′-TGATTGGTCTCACATGTTTGCT-3′
PAX1 5′-AACTTGGCTTAAATCTCTGCTCCAC-3′ 5′-GCATTGAAGGCTACATTTCACAGAC-3′
β-actin 5′-GTGGGGCGCCCCAGGCACCA-3′ 5′-CTTCCTTAATGTCAC GCACGATTTC-3′