Fig. 3
From: The establishment of a chemically defined serum-free culture system for human dental pulp stem cells
Colony-forming unit fibroblasts (CFU-F) of a serum-containing medium (SCM) and b E8. Statistical analysis of c CFU-F comparison (n = 5) and d bromodeoxyuridine (BrdU) proliferation assay (n = 5). BrdU fluorescence of hDPSCs in e E8 and f SCM. g Cell cycles were analyzed with FlowJo software. h Statistical analysis of the cell cycle (n = 5). i Cell proliferation analysis using the CCK-8 assay. The different optical density (OD) values are presented at 4 h, 24 h, 48 h, 72 h, 96 h, 120 h, and 6 days (n = 10). *p < 0.05, **p < 0.01