Fig. 6
From: The establishment of a chemically defined serum-free culture system for human dental pulp stem cells
Multi-differentiation analysis of hDPSCs. E8(left); SCM(right) a hDPSCs cultured in osteogenic medium for 15 days. b hDPSCs cultured in adipogenic medium for 15 days. c hDPSCs cultured in neurogenic medium for 3 h. d hDPSCs cultured in chondrogenic medium for 15 days. e Real-time polymerase chain reaction (PCR) analysis of the multidifferentiation capacities of hDPSCs. Relative quantities were measured, and significantly higher levels of expressions of OCN and RUNX2 (for osteogenic differentiation), MAP-2 (for neurogenic differentiation), and PPAR-γ (for adipogenic differentiation) were detected in E8 compared with serum-containing medium (SCM) (n = 5). *p < 0.05, **p < 0.01