Fig. 2
Tamoxifen treatment successfully ablates periosteal progenitors and their cilia in our transgenic animal models. Skeletally mature 16-week-old Prx1CreER-GFP;Rosa26DTA ablation mice and Prx1CreER-GFP controls received tamoxifen injections and were sacrificed a week later. Prx1-expressing cells (green) were observed in the periosteum of Prx1CreER-GFP control ulnae (a) but were absent in Prx1CreER-GFP;Rosa26DTA animals (b). Primary OCPs obtained from 3-week-old Prx1CreER-GFP;Ift88fl/fl animals (c) normally contain primary cilia (red); however, the presence and length of these cilia was diminished after exposure to 5 μM 4-hydroxytamoxifen (d). Indeed, cells treated with tamoxifen have decreased Ift88 mRNA expression (e). A magnified view of box region in panels c and d (f). Micrographs of the periosteum and isolated cells were collected at 20X and 40X, respectively. Nuclei are displayed in blue. Scale bars = 50 μm. Data are reported as mean and standard error. n = 4 for each group, ***p < 0.0001