Fig. 6

Platelet function analyses. Platelet function analyses. a Dose-dependent effect of adenosine on platelet activation (MFI of CD62p and PAC-1, n=3). b Phosporylation state of vasodilator-stimulated phoshoprotein (VASP), VASP-P MFI levels c Closure time measured as time platelets need to close an ADP/collagen-coated aperture measured using PFA-100 device, comparing nontreated, adenosine and cell-cocultured whole blood. ***p < 0.001. d Platelet function assessed using light transmission aggregometry (platelet aggregation profiler). Aggregation cascade can be separated into primary aggregation and slope, final aggregation (all left y axis) and area under the curve (AUC; right y axis) assessing entire aggregation response. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. ADP adenosine diphosphate, BM bone marrow, CB cord blood, HUVEC human umbilical vein endothelial cell, LA lipoaspirate, MFI mean fluorescence intensity, n/a not analyzed, PRP platelet-rich plasma, TRAP thrombin receptor activator for peptide, WB whole blood, w/o without