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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2

Fig. 1

NK92 cells were cotransfected with CRISPR/Cas and donor plasmid vectors targeting PRF1 gene. Cells selected in puromycin-containing media (3 mg/ml). Selected clones were screened for PRF1 locus and two positive clones designated clone 1 (a) and clone 2 (b) were employed in the study. Arrows show selected and proliferating colonies. No surviving cells visible in nontransfected control group (c). Phase-contrast microscopy, scale bar = 200 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512