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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells

Fig. 1

Similar expression of pluripotency markers in D3 and iICN1 ESCs. a Confocal immunofluorescence micrographs show expression of OCT4 (green), SSEA-1 (red), DAPI (blue), and merged images in D3 (upper panels) and iICN1 (lower panels) ESCs. Scale bars: 10 μm. b Western blot analysis of D3 and iICN1 ESCs with pluripotency markers (OCT4, NANOG, and SOX2) and GAPDH. c Expression level of ICN1 in iICN1 ESCs with (+) or without (−) Dox treatment for indicated time periods. Estimated molecular weight of ICN1 protein band indicated. Representative data from three independent experiments. DAPI 4′,6-diamidino-2-phenylindole, Dox doxycycline, GAPDH glyceraldehyde 3-phosphate dehydrogenase, iICN1 inducible intracellular domain of Notch1, M.W. molecular weight, SSEA-1 stage-specific embryonic antigen 1, SOX-2 sex determining region Y-box 2

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Stem Cell Research & Therapy

ISSN: 1757-6512