Fig. 3

JMJD6 depletion facilitates ADSCs proliferation. a Cell proliferation assay. The CCK8 assay was used to determine ADSCs cell number based on the standard curve. The absorbance of the cell suspension was measured at 450 nm. b Cell proliferation was assessed with an EdU labeling assay in JMJD6 depletion ADSCs and control cells. The images display EdU staining (red color) merged with Hoechst 33,342 staining (blue color). The graph represents as the percentage of EdU-positive cells. c. Cell cycle analysis. JMJD6 depleted ADSCs and control cells were incubated with propidium iodide staining solution and the cellular DNA contents were analyzed by flow cytometry. n = 3/group, Scale bar = 200 μm, magnification = ×100, Error bars represent the SD, **p < 0.01; *p < 0.05 versus the control group by the Student’s t test. ADSCs adipose-derived mesenchymal stem cells, EdU 5-ethynyl-2′-deoxyuridine, JMJD6 Jumonji C domain-containing protein 6, Sh1 ADSCs-JMJD6 sh1, Sh3 ADSCs-JMJD6 sh3, Scr ADSCs-JMJD6 scr