Fig. 5

JMJD6 regulates the expression of PDE1C by demethylating H4R3me2a at its promoter. a Western blot analysis of JMJD6, histone modifications, and PDE1C in JMJD6 knockdown ADSCs. b ChIP assays of JMJD6 and (c) H4R3me2a binding at the PDE1C promoter in JMJD6-depleted ADSCs or Scr control ADSCs. Primers used for the ChIP assays are shown as a schematic map at the top of the figures. d ChIP analysis of JMJD6 and H4R3me2a occupancies on regions near the PDE1C promoter in JMJD6- depleted and Scr control ADSCs. e Western blot analysis of JMJD6 and histone modifications in JMJD6 rescued cell lines. f ChIP assays of JMJD6 and H4R3me2a binding at the PDE1C promoter in JMJD6 rescue cell lines. Error bars represent the SD. TSS transcriptional start site. ADSCs adipose-derived mesenchymal stem cells, AMP cyclic hydrolysis both cyclic adenosine monophosphate, cGMP cyclic guanosine monophosphate, H4R3me2a histone dimethyl asymmetric H4R3, PDE1C phosphodiesterase 1C, JMJD6 Jumonji C domain-containing protein 6