Fig. 5

Mitochondrial respiration ability and glycolysis function in polycystic ovary syndrome (PCOS) patient-derived iPSCs. a Mitochondrial function based on the in-vitro oxygen consumption rate (OCR) in response to oligomycin, FCCP, rotenone, and antimycin (R/AA). b Glycolysis function based on the extracellular acidification rate (ECAR) in response to glucose, oligomycin, and 2-DG. c Quantitative analysis of basal oxygen consumption, ATP production, maximal respiration, and proton leak of iPSCs. d Quantitative analysis of glycolysis and glycolytic capacity of iPSCs. e Mitochondrial DNA (mtDNA) numbers normalized to nuclear DNA (nDNA). f Expression of mitochondrial RNA biogenesis gene PGC-1α. g Expression of mitochondrial RNA biogenesis related genes TFAM and NRF1. n = 3; error bars represent SD; *P < 0.05, **P < 0.01, versus non-PCOS (non-P)