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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Adipose tissue-derived extracellular fraction characterization: biological and clinical considerations in regenerative medicine

Fig. 4

The protective effect of adipose tissue extracellular fraction (AT-Ex) on cell viability following H2O2, UVA, and UVB irradiation. a AT-Ex and plasma supplementation were added at the concentration of 2% (v/v) immediately after exposure to stressor. Cell viability was determined 24 h post-treatment with UV or H2O2. The data represent the mean ± SD of 12 experiments performed in duplicate; statistical significance versus untreated control (Ctrl) is reported as *p < 0.05. b Death and apoptosis were evaluated by FACS analysis using annexin V/iodide propidium (PI) staining. Dot plots show one representative experiment performed 24 h after H2O2 or UVA exposure. The number of surviving cells (Annexin V/PI) was augmented in the presence of AT-Ex and plasma. OD optical density, NHF normal human fibroblasts, NHK normal human keratinocytes, NHM normal human melanocytes

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