Fig. 5
The effect of adipose tissue extracellular fraction (AT-Ex) on intracellular reactive oxygen species (ROS) clearing in normal human fibroblasts (NHF), normal human melanocytes (NHM), and normal human keratinocytes (NHK). a Cells were pretreated or post-treated with 2% (v/v) AT-Ex or matched plasma for 24 h and exposed to H2O2. Twenty-four hours after H2O2 treatment, cell viability was evaluated by MTT assay. Experiments were performed in triplicate; statistical significance versus untreated control (Ctrl) is reported as *p < 0.05 b The same experimental conditions described in (a) were used to quantify intracellular ROS levels using the fluorogenic probe H2DCF-DA by flow cytometric analysis. Histograms represent the mean ± SD of 14 (NHF) or 8 (NHK and NHM) independent experiments. c One representative histogram presenting the overlay of samples loaded with DCFH-DA. NHF blank = no probe loaded. OD optical density