Fig. 7
Partial reversal of cellular senescence by adipose tissue extracellular fraction (AT-Ex) supplementation in NHF. a, b After 72 h treatment, Western blotting using α-SMA and p21 antibodies and immunofluorescence analysis for IGFBP5 confirmed gene expression results. Images show representative data from three independent experiments. Similar results were obtained at 24 h (data not shown). NHF were incubated with 8-MOP for 4 h and then exposed to UVA (6 J/cm2) to induce premature senescence. Next, cells were grown in the presence (or not as control (Ctrl) cells) of 2% (v/v) AT-Ex (or 2% FBS for control cells) for 2 weeks. The acquisition of senescent phenotype was determined though the analysis of relevant senescence-associated markers at the mRNA (c) and protein level by ELISA (d) or Western bolt analysis (e). SA-β-gal assay confirmed the protective effect of AT-Ex (f). Quantitative data represent the mean ± SD of five independent experiments; statistical significance versus untreated control is reported as *p < 0.05