Fig. 8
Technical aspects of AT-Ex preparation. a Analysis of the effect of centrifugation versus a sedimentation step on NHF proliferation during the preparation of AT-Ex and plasma. b Evaluation of the impact of the filtration step during AT-Ex and plasma sample preparation on NHF fibroblast proliferation. c Evaluation of AT-Ex and plasma banking at low temperature (−80 °C). The biological effect of frozen versus not frozen AT-Ex and plasma were investigated measuring the impact on NHF proliferation or on cell viability following H2O2 exposure. Histograms represent the mean ± SD of six independent experiments; statistical significance versus untreated control (Ctrl) is reported as *p < 0.05. OD optical density