ELISA targets
|
AT-Ex
|
Plasma
|
Detection limit
|
---|
No. of positive samples
|
Mean ± SD
|
Range
|
No. of positive samples
|
Mean ± SD
|
Range
|
---|
bFGF
|
20/20
|
4430.7 ± 3102.9
|
1015.5–11476.0
|
0/20
|
ND
|
ND
|
50 pg/ml
|
EGF
|
20/20
|
35.3 ± 21.0
|
15.5–63.1
|
20/20
|
562.5 ± 186.1
|
160.8–772.6
|
26 pg/ml
|
Epo
|
20/20
|
5.24 ± 1.87
|
2.26–8.6
|
20/20
|
7.83 ± 4.9
|
3.0–16.9
|
0.17 mlU/ml
|
GM-CSF
|
17/20
|
2.17 ± 1.38*
|
01.6–4.4*
|
20/20
|
10.2 ± 11.0
|
2.0–26.6
|
0.6 pg/ml
|
KGF
|
17/20
|
486.3 ± 279.5*
|
176.4–1052.0*
|
8/20
|
602.4 ± 612.6*
|
150.0–1299.6
|
4.0 pg/ml
|
SCF
|
20/20
|
5.79 ± 2.82
|
1.6–10.4
|
0/20
|
ND
|
ND
|
0.45 ng/ml
|
NGF
|
20/20
|
95.2 ± 9.04
|
84.0–112.0
|
0/20
|
ND
|
ND
|
19.5 pg/ml
|
VEGF-A
|
20/20
|
2274.7 ± 964.7
|
315.8–2885.0
|
17/20
|
337.8 ± 243.3*
|
0–775.3*
|
7.9 pg/ml
|
α-MSH
|
20/20
|
2.16 ± 1.13
|
0.46–3.91
|
14/20
|
0.067 ± 0.043*
|
0.029–0.146
|
0.039 ng/ml
|
Wnt3a
|
20/20
|
21.6 ± 2.4
|
9.82–28.0
|
4/20
|
0.50 ± 0.45*
|
0.08 ± 1.25
|
0.054 ng/ml
|
Wnt10b
|
19/20
|
4.0 ± 1.26*
|
0.24–4.25
|
0/20
|
ND
|
ND
|
0.058 ng/ml
|
- bFGF basic fibroblast growth factor, EGF epidermal growth factor, ELISA enzyme-linked immunosorbent assay, Epo erythropoietin, GM-CSF granulocyte/macrophage colony-stimulating growth factor, KGF keratinocyte growth factor, MSH melanocyte stimulating hormone, ND not determined, NGF nerve growth factor, SCF stem cell factor, VEGF vascular endothelial growth factor, Wnt wingless type
- *mean values were calculated only with positive samples whereas range of concentration include negative sample (negative=0)