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Table 1 Determinations by ELISA for each factor analyzed, with the range of values observed among positive samples, the number of positive samples in each group, and the detection limit of assays

From: Adipose tissue-derived extracellular fraction characterization: biological and clinical considerations in regenerative medicine

ELISA targets

AT-Ex

Plasma

Detection limit

No. of positive samples

Mean ± SD

Range

No. of positive samples

Mean ± SD

Range

bFGF

20/20

4430.7 ± 3102.9

1015.5–11476.0

0/20

ND

ND

50 pg/ml

EGF

20/20

35.3 ± 21.0

15.5–63.1

20/20

562.5 ± 186.1

160.8–772.6

26 pg/ml

Epo

20/20

5.24 ± 1.87

2.26–8.6

20/20

7.83 ± 4.9

3.0–16.9

0.17 mlU/ml

GM-CSF

17/20

2.17 ± 1.38*

01.6–4.4*

20/20

10.2 ± 11.0

2.0–26.6

0.6 pg/ml

KGF

17/20

486.3 ± 279.5*

176.4–1052.0*

8/20

602.4 ± 612.6*

150.0–1299.6

4.0 pg/ml

SCF

20/20

5.79 ± 2.82

1.6–10.4

0/20

ND

ND

0.45 ng/ml

NGF

20/20

95.2 ± 9.04

84.0–112.0

0/20

ND

ND

19.5 pg/ml

VEGF-A

20/20

2274.7 ± 964.7

315.8–2885.0

17/20

337.8 ± 243.3*

0–775.3*

7.9 pg/ml

α-MSH

20/20

2.16 ± 1.13

0.46–3.91

14/20

0.067 ± 0.043*

0.029–0.146

0.039 ng/ml

Wnt3a

20/20

21.6 ± 2.4

9.82–28.0

4/20

0.50 ± 0.45*

0.08 ± 1.25

0.054 ng/ml

Wnt10b

19/20

4.0 ± 1.26*

0.24–4.25

0/20

ND

ND

0.058 ng/ml

  1. bFGF basic fibroblast growth factor, EGF epidermal growth factor, ELISA enzyme-linked immunosorbent assay, Epo erythropoietin, GM-CSF granulocyte/macrophage colony-stimulating growth factor, KGF keratinocyte growth factor, MSH melanocyte stimulating hormone, ND not determined, NGF nerve growth factor, SCF stem cell factor, VEGF vascular endothelial growth factor, Wnt wingless type
  2. *mean values were calculated only with positive samples whereas range of concentration include negative sample (negative=0)