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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Single-molecule fluorescence in-situ hybridization reveals that human SHANK3 mRNA expression varies during development and in autism-associated SHANK3 heterozygosity

Fig. 1

SHANK3 mRNA and protein puncta selection in hiPSC-derived cortical neurons. a Left panel: bright-field images used to manually outline cell body and neuronal processes. Right panel: puncta (circled) detected using automated algorithm, defined by intensity and size threshold. Only puncta lying within outlined areas included. b Puncta detected at each plane (1-ÎĽm intervals) per Z-stack. SHANK3 SH3 and multiple ankyrin repeat domains 3

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Stem Cell Research & Therapy

ISSN: 1757-6512