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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Single-molecule fluorescence in-situ hybridization reveals that human SHANK3 mRNA expression varies during development and in autism-associated SHANK3 heterozygosity

Fig. 2

SHANK3 mRNA and protein labelling and co-localization in hiPSC-derived neurons. a Immunofluorescence labelling of SHANK3 protein (red) in MAP2-positive dendrites (blue) of hiPSC-derived neurons. Arrows mark co-localization of SHANK3 protein. b SHANK3 (green) co-localizes with the pre-synaptic marker synapsin (blue) and post-synaptic marker PSD95 (red). Arrowheads mark co-localization of SHANK3 protein. c Schematic illustrating SHANK3 mRNA. Vertical red lines indicate complementary binding sites for smFISH probes along both coding domain and 3′-UTR. SHANK3 antibody binding site marked green. d Representative Z-stack of single SHANK3 mRNA transcripts labelled by smFISH in 51 DIV hiPSC-derived neurons. SHANK3 mRNA localized to cell body and processes in hiPSC-derived neurons. e Combined SHANK3 mRNA and protein labelling in 49-DIV hiPSC-derived neuron. White arrowheads label SHANK3 protein puncta. White arrows label SHANK3 mRNA puncta. Ab antibody, FISH fluorescence in-situ hybridization, MAP2 microtubule-associated protein 2, SHANK3 SH3 and multiple ankyrin repeat domains 3, UTR untranslated region

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Stem Cell Research & Therapy

ISSN: 1757-6512