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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea

Fig. 1

Identification of induced pluripotent stem cells (iPSCs) formed from urinary cells. a Alkaline phosphatase staining of iPSCs. Endogenous expression of SOX2, KLF, OCT4, and c-MYC in iPSCs compared with that in urinary cells. Scale bars = 100 μm. b Formation of embryoid bodies (EBs) in suspension culture of iPSCs. EBs expressed AFP, CK8, and CK18 specific for endoderm, MSX1 and Brachyury specific for mesoderm, and PAX6 and SOX1 specific for ectoderm. Scale bars = 100 μm. c Hematoxylin and eosin staining of teratoma derived from iPSCs in NOD/SCID mouse displaying various structures, including gut epithelium in endoderm, neural rosettes and retinal pigment in ectoderm, and cartilage in mesoderm. d Immunostaining for pluripotent markers OCT4, NANOG, TRA-1-81, TRA-1-60, and SSEA4 in iPSCs. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; blue). Scale bars = 200 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512