Fig. 1

Chronological macroscopic and microscopic changes of the human ovary during SLES-based decellularization process. a-d The color of the bisected ovarian samples turned from red to white while the samples preserved their shape and homogeneity. e A lyophilized decellularized ovarian scaffold with visible pores once populated by growing follicles; scale bars: 10 mm. g-j Hematoxylin and eosin (i and j), and Hoechst (g and h) staining of intact (g and i) and decellularized (h and j) ovary showed it was devoid of nucleic materials. f A drastic decrease in DNA content after decellularization. (Data are expressed as the mean ± standard error of the mean (SEM), N = 3 per group, *Indicates significant difference, p = 0.0001)