Fig. 3

miR-431 targets the 3′-UTR of IRS2. a The mRNA expression levels of insulin receptor substance 2 (IRS2) in hMSCs infected by miR-431 lentivirus or negative control (NC) lentivirus. b The protein expression levels of IRS2 in hMSCs infected by miR-431 lentivirus or NC lentivirus. c The matching of miR-431 with IRS2 3’-untranslated regions (UTRs). d Luciferase activity assay; HEK293 cells were infected by miR-431 lentivirus or NC lentivirus and transfected with wild-type (WT) or mutant (MUT) IRS2 3′-UTR reporter. Data are presented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, versus NC