Fig. 2
Secretome characterization and test data. a Unsorted MSCs were expanded on all PDMS substrata of varying stiffness (1, 10, and 100 kPa PDMS) for 7 days, represented by light, medium, and dark orange colors, respectively.) Expression of MSC secreted proteins that have significant trends (§p < 0.001) with cell culture substratum stiffness as determined from Spearman’s rank-based correlation. b As a comparison to MSCs on PDMS, unsorted and Dhi MSCs were also grown on TCPS and are represented by striped purple and blue bars, respectively. Expression of MSC secreted proteins identified in Table 1 were assayed across all five different culture conditions. Expression of MSC secreted proteins that have significant trends (*p < 0.05, **p < 0.01, ***p < 0.005) with cell culture substratum stiffness as determined from Spearman’s rank-based correlation. All data are plotted as arithmetic mean ± standard deviation