Fig. 1

Single cell sequencing flow chart. Brain tissues from the brain region of interest are collected, then single cells are captured by fluorescence-activated cell sorting (FACS), laser capture microdissection (LCM), and microfluidics (Fluidigm C1 and 10× Genomics Chromium). Once single cells are captured, DNA or RNA are amplified for the next sequencing step. Principle component analysis (PCA) and hierarchical clustering are ideal tools to analyze the single cell sequencing data and to classify cell types