Fig. 3

Two methods to add barcode in a single cell. a cDNA is reverse-transcribed and amplified using the oligo dT primer (green) in the presence of a template-switching oligonucleotide that contains a unique molecular identifier (UMI; red) and a PCR primer for Illumina sequencing (brown). After amplification, tagmentation is performed using Tn5 transposase carrying a specific cell barcode (blue) and a PCR primer. b cDNA is reverse-transcribed and amplified using an oligonucleotide that consists of oligo dT, UMI, a cell barcode, and a PCR primer, and then tagmented after amplification. P1 and P2 are PCR primers for establishing libraries for Illumina sequencing