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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Revealing cellular and molecular complexity of the central nervous system using single cell sequencing

Fig. 5

Whole transcriptome amplification methods for single cell sequencing. a Oligo dT-anchor approach. mRNA is reverse-transcribed with an oligo-dT anchored primer, and poly(A) is added to the 3′ end of the first-strand cDNA to synthesize the second strand cDNA. b Template-switching approach. mRNA is reverse-transcribed by Moloney murine leukemia virus (MMLV) reverse transcriptase, and a few non-templated C nucleotides are added to the 3′ end of the first-strand cDNA to synthesize the second strand cDNA

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Stem Cell Research & Therapy

ISSN: 1757-6512