Fig. 4

AD increases IL-1β secretion by activating NF-κB signaling pathway. a Activation of NF-κB in PCa cells after AD administration detected by western blot analysis of p-IκBα and IκBα. b Western blot analysis of NF-κB activation in tumor tissues of mice suffering castration. c Inhibition of IκBα phosphorylation by BAY11-7082 (10 μM) confirmed using western blot analysis. d RT-PCR analysis of IL-1β expression level in LNCaP cells after AD administration, in absence and presence of BAY11-7082. e ELISA performed to examine concentration of IL-1β in CM isolated from LNCaP cells after AD administration, in absence and presence of BAY11-7082. f MSC migration assays performed after adding BAY11-7082 to lower chamber when PCa cells suffered from AD. *p < 0.05, **p < 0.01. AD androgen deprivation, BAY11-7082 NF-κB inhibitor, DMSO dimethylsulfoxide, IL interleukin, MSC mesenchymal stem cell