Fig. 7
The fate of transplanted muscle-derived cells (MDCs) in the submucosal layer. Images of cross-section after immunohistochemical staining. 1,1′-Dioctadecyl-3,3,3,-tetrametlylindodicarbocyanine-5,5-disulfonic acid (DID)-labeled transplanted MDCs are red, AF488-labled desmin is green, 4',6-diamidino-2-phenylindole (DAPI)-labeled nuclei are blue. a Wide view of the submucosal region with a DID-positive depot; (b–e) Represent the same field of view (corresponding to the area marked on the neighboring picture). Numerous DIDneg/desminpos mononulear cells can be observed around the DIDpos depot. Scale bar (a), 200 μm