Fig. 8
The contribution of transplanted muscle-derived cells (MDCs) and mesenchymal stem/stromal cells (MSCs) to the blood vessel formation. Images of cross-section after immunohistochemical staining. 1,1′-Dioctadecyl-3,3,3,-tetrametlylindodicarbocyanine-5,5-disulfonic acid (DID)-labeled transplanted MDCs are red, AF488-labeled desmin is green, 4',6-diamidino-2-phenylindole (DAPI)-labeled nuclei are blue. (A) urethra from the MDC group. Arrowhead indicates the DIDpos/desminpos muscle fiber cross-section. B, B′, B″, B″‘ represent the same field of view (corresponding to the area marked on the neighboring picture). The contribution of DIDpos cells to the blood vessel wall formation can be observed. (C) Urethra from the MSC group. D,D’,D″,D″‘ represent the same field of view (corresponding to the area marked on the neighboring picture). The contribution of DIDpos cells to the blood vessel wall formation can be observed (marked with the arrow). Scale bars, (A) 200 μm, (C) 50 μm