Fig. 1

IL-10 recapitulates adipose-derived mesenchymal stem cell-dependent expansion of normal human breast epithelial progenitors. a Experimental outline for adipose-derived mesenchymal stem cell (AdMSC)-dependent expansion of human breast epithelial progenitors (HBEPs) in organoid cultures. b AdMSC effect on total progenitor (colony forming cell (CFC) numbers) yield in organoid cultures initiated with normal human breast epithelial cells (HBECs). c AdMSC effect on frequency of luminal and bipotent CFCs. AdMSC effect on luminal (d) and bipotent (e) CFC yields in organoid cultures initiated with luminal or bipotent CFC-enriched subsets of HBECs. f Experimental outline for cytokine ELISA array performed on conditioned media (CM) obtained from organoid cultures initiated with lineage depleted (Lin⁻) EpCAM⁺ HBECs, AdMSCs, or cocultures of both cell types. g ELISA array identified four candidate cytokines upregulated in coculture CM compared to AdMSC-alone CM. All cytokine levels normalized to HBEC CM (= 1). Lin⁻ HBECs placed in organoid cultures and treated with different cytokines and (h) average cell numbers and (i) CFC yields determined. j Effect of recombinant IL-10 on relative yields of luminal and bipotent CFCs. Effect of IL-10 on (k) luminal and (l) bipotent CFC yields in organoid cultures initiated luminal or bipotent CFC-enriched fractions. All results are represented as means ± SEM from at least three biological replicates. Cont. control, 2D two-dimensional, 3D three-dimensional, GRO growth-regulated oncogene, IL-10, interleukin 10, MCP3 monocyte chemotactic protein 3, MSC mesenchymal stem cell, RANTES regulated on activation, normal T cell expressed and secreted. *p < 0.05, **p < 0.005, ***p < 0.0001, ****p < 0.00001