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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Gene expression profiling of chondrogenic differentiation by dexamethasone-conjugated polyethyleneimine with SOX trio genes in stem cells

Fig. 3

Evaluation of the expression level of chondrogenesis-related markers at various time points after the transfection of hMSCs. a Brief schematic of the 3D culture of hMSCs following the transfection of differentiation-inducing nanoparticles (DI-NPs). b RT-PCR (panel a) and Western blot analysis (panel b) of chondrogenesis-related markers in hMSCs transfected with DI-NPs. c Immunohistological analysis of hMSCs at 7 days (panels a–e), 14 days (panels f–j), and 21 days (panels k–o) after the transfection of DI-NPs. n = 5. COL collagen, MMP matrix metallopeptidase

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