Fig. 6
Effect of exosomes on CCSMC apoptosis. a The primary CCSMCs emerging from corporal tissue (left) and the passaged cells growing in a whirlpool-like pattern (right), at ×100 magnification. b Immunofluorescence with anti-calponin antibody for CCSMC identification, ×100 amplification. c Flow cytometric analysis of cell viabilities. Cells were cultured in the absence (0 μg/ml) or presence (10 μg/ml, 20 μg/ml) of MSC-Exos followed by H2O2 treatment. d The average apoptotic cell percentages are shown for cells cultured in the absence (0 μg/ml) or presence (10 μg/ml, 20 μg/ml) of MSC-Exos. All values are represented as the mean ± SD (*p < 0.05; n = 3). e. Western blot analysis of expression of caspase-3 in CCSMCs cultured in the absence (0 μg/ml) or presence (10 μg/ml, 20 μg/ml) of MSC-Exos followed by H2O2 treatment. f Data are presented as the relative density of caspase-3 compared with that of β-actin. All values are represented as the mean ± SD (*p < 0.05; n = 3)