Fig. 3
Assay for evaluating the fate of intraperitoneally injected hAT-MSCs. A serial dilution of 1 × 106 hAT-MSCs was injected in mice to investigate the expression level of human/mouse GAPDH and human-specific GAPDH. a Standard curves for quantitative reverse transcription polymerase chain reaction assay of human-specific mRNA levels of GAPDH. b, c, d, e Distribution of hAT-MSCs in seven organs 2, 12, 24, and 48 h after their intraperitoneal infusion. The results are representative of three independent experiments. GAPDH glyceraldehyde-3-phosphate dehydrogenase, hAT-MSCs human adipose tissue-derived mesenchymal stem cells, I.P. intraperitoneal