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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Human cardiac stem cells inhibit lymphocyte proliferation through paracrine mechanisms that correlate with indoleamine 2,3-dioxygenase induction and activity

Fig. 3

Human cardiac/stem progenitor cells (hCSCs) inhibit T lymphocyte proliferation in a time- and hCSC concentration-dependent manner. CFSE-labeled hPBMCs were stimulated with PHA and cultured alone or in direct contact with hCSCs (ratios 1:10, 1:17, and 1:25 hCSCs:hPBMCs). After 72 h (white bars) and 96 h (black bars), proliferation of the viable population of CD3-viable T lymphocytes (CD3+/7AAD) was assayed by loss of CFSE staining. Percentage of inhibition of proliferation was determined using FSC Express software against proliferation of activated hPBMCs alone. Human adipose-derived mesenchymal stem cells (hASCs) were used as a positive control for T cell proliferation inhibition (ratio 1:25 hASCs:hPBMCs). Adjusted p values are shown

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