Fig. 2

The culture systems used in this study to culture monocytes (THP-1) alone or with H₂O₂ or with DBMSCs and H₂O₂ or with HUVEC and H₂O₂. Monocytes (THP-1) cultured alone in RPMI-1640 culture medium (a) or with 100 μM H₂O₂ (b) or with DBMSCs (physical contact experiment) and 100 μM H₂O₂ (c), HUVEC cultured with THP-1 pretreated with DBMSCs and 100 μM H₂O₂ (Physical contact experiment) in a complete endothelial cell growth medium in the presence of 100 μM H₂O₂ (d). THP-1 were cultured with DBMSCs at different THP-1:DBMSC ratios (2.5:1, 5:1, 10:1 and 20:1 THP-1:DBMSC) in the presence or absence of 100 μM H₂O₂. HUVEC were cultured with THP-1- pre-treated with DBMSCs and H₂O₂ at different THP-1:HUVEC ratios (2.5:1, 5:1, 10:1 and 20:1 THP-1:HUVEC) in the presence or absence of 100 μM H₂O₂. Cells were then incubated for 96 h at 37 °C in a cell culture incubator