Fig. 1

Characterization of in vitro-cultured FD-derived cells. a FP and RM cells (isolate 2) cultured with 100 ng/mL BMP-2 for 6 days exhibited increased ALP activity relative to their respective control whereas PP cells (isolate 2) did not. Semi-quantification of ALP activity in FP, PP, and RM cells. b FP and PP cells (isolate 2) cultured with 100 ng/mL BMP-2 and 100 nM dexamethasone for 24 days did not exhibit increased Alizarin Red S staining relative to their respective control. Quantification of Alizarin Red S staining in FP and PP cells. c FACS analysis of RM cells. The percentage of cells that were negative for STRO1 and ALP, negative for STRO1 but positive for ALP, negative for ALP but positive for STRO1, and positive for both STRO1 and ALP were 0.28–1.25%, 91.83–97.53%, 0.004–0.10%, and 1.20–7.89%, respectively. STRO1 and ALP immunofluorescence staining in RM cells. Green, STRO1-positive cells. Red, ALP-positive cells. Scale bars as indicated. Data were from n = 3 isolates (three independent experiments with nine replicates per isolate for ALP and mineralization studies and one independent experiment with three replicates per isolate for FACs studies). Gray circles indicate observed data points. Error bars indicate standard error of mean or SEM. Statistical significance as indicated