Fig. 3

RNA-seq analysis of RM cells and hMSCs under proliferation and mineralization conditions. a RNA-seq analysis of RM cells (isolate 2) and hMSCs (isolate 24268) under serum-free (0% serum) and serum-containing (10% serum) conditions identified 40 candidate proliferation genes. Scatterplots indicate the correlation (r²) between replicates for each condition. FPKM, fragments per kilobase of transcript per million mapped reads. b Gene ontology enrichment analysis of RM cells (isolate 2) and hMSCs (isolate 24268) under proliferation conditions. Graphs indicate the top 5 upregulated (red) and downregulated (green) biological processes, cellular components, and molecular functions. Data were from n = 1 isolate (one independent experiment with two replicates per group)