Fig. 6

IGF-1 knockdown decreased apoptosis. a Representative terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) images of BM-MSCs treated with H/SD with or without IGF-1 siRNA (scale bars, 20 μm). b The quantification result of the apoptotic BM-MSCs with or without IGF-1 siRNA. c Representative results of the FACS analysis of BM-MSCs under normal and H/SD conditions with or without IGF-1 siRNA administration. d Quantification of apoptosis is presented as the percentage of cells with the annexin marker in the early and late apoptotic stages with or without IGF-1 siRNA. Data are expressed as the means ± SEM; n = 5; *p < 0.05