Fig. 8

IGF-1 knockdown protects aged BM-MSCs by increasing autophagy. a Representative terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) images of aged BM-MSCs treated with IGF-1 siRNA with or without autophagy inhibition via 3-methyladenine (3-MA) and Atg7 small interfering RNA (siRNA). b Representative terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) images of aged BM-MSCs treated with IGF-1 siRNA with or without autophagy inhibition via 3-methyladenine (3-MA) and Atg7 small interfering RNA (siRNA) under normal and hypoxic conditions. c Quantification of apoptotic BM-MSCs under normal and hypoxic conditions. d Representative immunofluorescence images of GFP-LC3 (green fluorescent) and DAPI (blue fluorescence) in BM-MSCs of each group (scale bars, 20 mm). e Histogram showing the percentages of BM-MSCs with punctate LC3 in each group under normal and hypoxic conditions