Fig. 6

p38 MAPK signaling pathway is involved in CXCR3 protein expression in MSCs, CXCR3-mediated chemotaxis invasion, and transendothelial migration induced by IL-1β. a MSCs were pretreated with p38 MAPK inhibitor SB203580, at a concentration of 25 uM, then stimulated with IL-1β for 30 min. The expression of CXCR3 cytosolic and membrane protein were detected by Western blotting. b Quantitative results of the Western blotting of CXCR3 cytosolic and membrane protein expression of a. The data represent mean ± SD (n = 3). Statistical analysis was determined by Student’s t test and one-way ANOVA. *P < 0.05, ***P < 0.001. c MSCs were pretreated or non-pretreated with SB203580 and stimulated with or without IL-1β for 30 min. After pre-activation, MSCs were plated on agarose drop supplemented with the presence or absence of 50 ng/ml CXCL9 for 24 h. Scale bars = 500 μm. d Quantitative results showing the chemotaxis invasion ability of MSCs migrated under agarose drop. The data represent mean ± SD (n = 3). Statistical analysis was determined by Student’s t test and one-way ANOVA. **P < 0.01, ***P < 0.001. e MSCs were pretreated or non-pretreated with p38 MAPK inhibitor and stimulated with or without IL-1β for 30 min. After pre-activation, MSCs were plated on activated or non-activated HUVEC monolayers in the upper chamber and incubated in the presence or absence of 50 ng/ml CXCL9 in the lower chamber for 16 h. Scale bars = 300 μm. f Quantitative results showing the transendothelial migration ability of MSCs migrated to the lower chamber. The data represent mean ± SD (n = 3). Statistical analysis was determined by Student’s t test and one-way ANOVA. **P < 0.01, ***P < 0.001